p53-induced apoptosis - 輔仁大學 理工學院 生物技術研發中心 ... 、大腸癌和乳癌有 p53 的 mutation,其位在 chromosome 17 上,p53 protein 由 383 個 amino acid 組成,會影響 cell cycle ... 當放 p53 時,sub-G1 升高了,所以說有很多的細胞已經開始凋亡,當放入 Mdm2 時凋亡的細胞減少了,從原本的 ...
DNA fragmentation and Apoptosis - Flow Cytometry Core Facility ... S-phase and G2M will be observed with apoptotic cells being represented by a sub G0/G1 population seen to the left of the G0/G1 peak. ...
中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/1108 sub-G1(apoptosis)的表現量顯著的增加,DNA斷裂情形也被觀察到(6.25,12.5, 25μg ... analysis showed that Pipoxolan caused p53- mediated apoptosis by enhanceing p21 then decreasing cyclin D1 to arrest the cell cycle in G0/G1 phase. It ...
DNA fragmentation and Apoptosis - Flow Cytometry Core Facility DNA fragmentation and Apoptosis at the Flow Cytometry Core Facility, Institute ... However in order to be seen in the SubG1 area, a cell must have lost enough ...
SubG1 Analysis Using Propidium Iodide Flow Cytometry Core Facility, Camelia Botnar Laboratories, Room P3.016. UCL Institute of Child ... can be counted in subG1 region of PI histogram. Materials. 1.
細胞週期及DNA 分析(PDF) Flow Cell. Waste. Argon Ion Laser. ( 488 nm). Basics of Flow cytometry. Light Scatter ... Cell Cycle : FL3 Lin. Flow Raw Data ... cells : 亞二倍體峰(Sub-G1 phase ) ...
Does sub-g1 peak in cell cycle analysis (flow cytometry w/ propidium ... 20 Feb 2014 ... When I use a cleaved caspase-3 kit for detecting apoptosis, I haven't the same percentage of apoptotic cells as it is in sub G1 with a flow ...
Can apoptosis be detected by cell cycle studies? - ResearchGate 29 Aug 2012 ... Agree with Despina that cell cycle with PI staining is not the precise way to quantify the extent of apoptosis. On additional reason is that sub-G1 ...
Chapter 9: Cell Death, Including Apoptosis - Flow Cytometry - A ... 12 Aug 2010 ... These cells can be observed as a hypodiploid or 'sub-G1' peak in a DNA ... The cells had undergone apoptosis from G1 of the cell cycle.
Cell cycle / sub-G1 peak apoptosis cell cycle/subg1 etc -apoptosis. Geert Martens wrote: >we have done a series of experiments with mitochondrial poisons (such as >rotenone) in rat pancreatic ...